六苄基六氮杂异伍兹烷对大鼠的致畸性研究

夏洁芳; 高俊宏; 刘志永; 王鸿; 岳红; 高永超; 孙成辉; 宋建伟

doi:10.13421/j.cnki.hjwsxzz.2016.04.004

六苄基六氮杂异伍兹烷对大鼠的致畸性研究

Study on the Teratogenicity of Hexa-benzyl-hexa-aza-iso-wurtzitane in Rats

摘要

摘要:
目的开展大鼠致畸试验，探索六苄基六氮杂异伍兹烷(Hexabenzylhexaazaisowurtzitane，HBIW)职业接触对育龄妇女妊娠的潜在影响。
方法选取SPF级性成熟的SD大鼠，雌性88只，雄性60只，雌雄合笼交配，将孕鼠随机分成HBIW低（111 mg/Kg·bw）、中（333 mg/Kg·bw）、高（1 000 mg/Kg·bw）3个剂量组、阴性对照组，每组22只，于孕期第5 d~19 d经口给予受试物，观察孕鼠和胎鼠的毒性和致畸性。
结果各试验组及阴性对照组在黄体数、着床率、活胎数、死胎数、吸收胎数、胎盘数及子宫连窝重上差别尚不能认为有统计学意义（P>0.05）；阴性对照组和三个剂量组全部胎鼠未检查出任何畸形，四组间性别构成比无显著性差异（P>0.05）。HBIW在本实验剂量条件下的致畸指数约等于5。
结论在本实验条件下，HBIW各剂量组未发现对大鼠有致畸毒性和母体毒性。

Abstract:
Objective To investigate the potential impact of occupational exposure to Hexa-benzyl-hexa-aza-iso-wurtzitane (HBIW) on women of childbearing age.
Methods Eighty eight pregnant SD rats were divided into four groups, one negative control group and three exposure groups. HBIW was administered by gavage to 3 exposure groups from the day 5 to day 19 during pregnancy at the dosage of 111mg/kg·bw, 333 mg/kg·bw and 1 000 mg/kg·bw. Body weight gain of pregnant rats, body weight, body length and tail length of live fetus and some other parameters affecting embryonic development were measured.
Results Corpus luteum counts, nidation counts, vivifetus counts, the number of dead and reabsorbed fetus in 3 exposure groups were not significantly different from those in the negative control group (P>0.05). No deformity was observed in the fetus of all groups, the constituent ratio of all groups were not significantly different(P>0.05). The teratogenic index of HBIW was approximately equal to 5.
Conclusion Teratogenic toxicity and maternal toxicity were not observed in rats exposed to HBIW.

HTML全文

参考文献(7)

施引文献

资源附件(0)