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    李刚强, 焦成文, 周玉坤. 60Coγ亚致死量辐射致小鼠外周血中MicroRNA表达改变[J]. 环境卫生学杂志, 2016, 6(1): 28-31. DOI: 10.13421/j.cnki.hjwsxzz.2016.01.007
    引用本文: 李刚强, 焦成文, 周玉坤. 60Coγ亚致死量辐射致小鼠外周血中MicroRNA表达改变[J]. 环境卫生学杂志, 2016, 6(1): 28-31. DOI: 10.13421/j.cnki.hjwsxzz.2016.01.007
    shu
    LI Gangqiang, JIAO Chengwen, ZHOU Yukun. Differential Expression Profile of MicroRNA in Mice Peripheral Blood Induced by Sublethal Dose of Irradiation[J]. Journal of Environmental Hygiene, 2016, 6(1): 28-31. DOI: 10.13421/j.cnki.hjwsxzz.2016.01.007
    Citation: LI Gangqiang, JIAO Chengwen, ZHOU Yukun. Differential Expression Profile of MicroRNA in Mice Peripheral Blood Induced by Sublethal Dose of Irradiation[J]. Journal of Environmental Hygiene, 2016, 6(1): 28-31. DOI: 10.13421/j.cnki.hjwsxzz.2016.01.007
    shu

    60Coγ亚致死量辐射致小鼠外周血中MicroRNA表达改变

    Differential Expression Profile of MicroRNA in Mice Peripheral Blood Induced by Sublethal Dose of Irradiation

      摘要
    • 摘要: 目的 探讨60Coγ射线亚致死量辐射损伤对小鼠外周血中MicroRNA的影响及意义。方法 SPF级C57BL/6J小鼠接受2Gy全身照射后,分别于照射后6、24和72 h进行外周血白细胞计数。同时应用Agilent MicroRNA生物芯片筛选小鼠血中差异表达MicroRNA。结果 MicroRNA芯片筛选出照射后6 h差异表达MicroRNA共16个,其中13个上调,3个下调; 24 h后差异表达MicroRNA共36个,其中13个上调,23个下调; 72 h后差异表达MicroRNA共30个, 其中13个上调,17个下调。与未照射组比较,表达差异有统计学意义(P<0.05)。筛选15个MicroRNA组成的表达谱,对辐射损伤时间的判断有很好的效果,准确率90%以上。结论 MicroRNA在血中差异表达与辐射损伤有关,其有望成为由外周血检测辐射损伤的新标志物。

       

      Abstract: Objectives To investigate the differential expression profiles of MicroRNA in peripheral blood of mice induced by sublethal dose of irradiation and to explore their major effects and significances.Methods SPF C57BL/6J mice were exposed to a single 2Gy whole body irradiation with60Coγ, the changes of MicroRNA expression in peripheral blood were detected by Agilent MicroRNA microarrays at 6, 24and 72h after irradiation.Results At 6h after irradiation with 2Gy of 60Coγ, the total number of differentially expressed MicroRNA was 16, in which 13 was up-regulated and 3 down-regulated. At 24 h and 72 h after irradiation, 36 MicroRNA and 30 MicroRNA were differentially expressed, respectively.Conclusions MicroRNA was involved with the regulation of sublethal dose radiation damage, and the differential expression of MicroRNA in peripheral blood of mice derived from sublethal dose irradiation might be used as a new biomarker for the exposure of sublethal irradiation.

       

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