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    杨拉维, 王亚红, 杨腾, 陈婷, 何惠娟, 刘刚. PM2.5对A549细胞IL-1β表达的影响[J]. 环境卫生学杂志, 2015, 5(6): 493-497. DOI: 10.13421/j.cnki.hjwsxzz.2015.06.001
    引用本文: 杨拉维, 王亚红, 杨腾, 陈婷, 何惠娟, 刘刚. PM2.5对A549细胞IL-1β表达的影响[J]. 环境卫生学杂志, 2015, 5(6): 493-497. DOI: 10.13421/j.cnki.hjwsxzz.2015.06.001
    shu
    YANG Lawei, WANG Yahong, YANG Teng, CHEN Ting, HE Huijuan, LIU Gang. Effect of PM2.5 on IL-1β Expression in A549 Cells[J]. Journal of Environmental Hygiene, 2015, 5(6): 493-497. DOI: 10.13421/j.cnki.hjwsxzz.2015.06.001
    Citation: YANG Lawei, WANG Yahong, YANG Teng, CHEN Ting, HE Huijuan, LIU Gang. Effect of PM2.5 on IL-1β Expression in A549 Cells[J]. Journal of Environmental Hygiene, 2015, 5(6): 493-497. DOI: 10.13421/j.cnki.hjwsxzz.2015.06.001
    shu

    PM2.5对A549细胞IL-1β表达的影响

    Effect of PM2.5 on IL-1β Expression in A549 Cells

      摘要
    • 摘要:
      目的  研究6个不同城市大气污染颗粒PM2.5对肺癌A549细胞炎症因子IL-1β的影响, 探讨PM2.5成分和炎症因子IL-1β之间的关系。
      方法  采集湛江、广州、武汉、上海、北京和成都6个城市PM2.5, 测定不同城市PM2.5中金属元素及多环芳烃(PAHs)的含量。将不同城市PM2.5以100 μg/mL染毒A549细胞24 h, 荧光定量PCR检测不同城市PM2.5染毒细胞后细胞内炎症因子IL-1β基因表达水平。通过线性回归和相关性分析研究PM2.5成分和细胞炎症因子之间的关系。
      结果  6个城市PM2.5中均含有高浓度的PAHs, 其中广州、武汉、上海3个城市PM2.5中PAHs浓度高达900 pg/μg, 高于其他3个城市。6个城市PM2.5金属成分中, 过渡金属元素Fe、Cu、Ni含量较高。6个城市PM2.5均能够引起A549细胞内IL-1β基因表达水平上调, PM2.5中PAHs和Ni元素含量与细胞内IL-1β表达水平呈显著相关。
      结论  不同城市的PM2.5能上调细胞IL-1β的表达, PM2.5上调细胞内IL-1β的作用与PAHs和Ni元素相关。

       

      Abstract:
      Objectives  To investigate the effects of PM2.5 on the expression of inflammatory factor IL-1β in lung cancer cell line (A549 cells) and the associations between PM2.5 composition and the expression of IL-1β.
      Methods  The concentration of metals and PHAs in PM2.5 collected from six cities, Zhanjiang, Guangzhou, Wuhan, Shanghai, Beijing and Chengdu were analyzed.A549 cells were exposed to PM2.5 at a dose of 100 μg/mL for 24 h; and the expression of IL-1β in A549 cells was tested by Q-PCR.The association between the composition of PM2.5 and the expression of IL-1β was assessed by using linear regression and correlation analysis.
      Results  The content of PAHs in PM2.5 from Guangzhou, Wuhan and Shanghai was about 900 pg/μg, which was higher than that in other three cities.The concentration of Fe, Cu, and Ni was very high in all PM2.5 samples, and these transition metals could induce the expression of IL-1β in A549 cells.High expression of IL-1β was strongly associated with high concentrations of PAHs and Ni in PM2.5.
      Conclusions  PM2.5 collected from 6 cities could induce the expression of IL-1β in A549 cells.The upregulation of IL-1β in A549 cells induced by PM2.5 was associated with the high PAHs and Ni content in PM2.5.

       

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