Abstract:
Objectives To investigate the cytotoxicity of nanometer copper-oxide on CHO cells in continuous time points to obtain accurate data as a reference for the research on toxicological mechanism in following studies.
Methods The growth of CHO cells with different cell densities were determined continuously by RTCA(real-time cell analysis). The cytotoxicity of nanometer copper-oxide with different concentration on CHO cells was tested by real-time monitoring; then the IC50 value at different time points could be obtained to make a curve. All of these results could be used to detect the cytotoxicity of nanometer copper-oxide on CHO cells.
Results The logarithmic growth of cells at different time points for four cell densities was different, and the density of cells in 5×103/hole was the best for testing the effect of toxicity. The toxic effect was started at about 4~5 h after treatment with different doses of nanometer copper-oxide, and the IC50 value after 12, 24, 36 and 48 h of treatment was 0.249, 0.207, 0.236 and 0.412 mg/mL, respectively.
Conclusions The toxic effect of nanometer copper-oxide on CHO cells was started at about 3 h after treatment, and the IC50 value was declined dramatically after treated for 6 h, and declined with the extension of time. After the treatment of nanometer copper-oxide for 18 h, the IC50 value was less than 0.2 mg/mL continuously.
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