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    姜瑞雪, 牛志尊, 黄密, 夏婷婷, 马作峰. 杏苏散、桑杏汤对PM2.5染毒大鼠肺组织中HMGB1、TNF-α、IL-6表达的影响[J]. 环境卫生学杂志, 2015, 5(4): 317-320. DOI: 10.13421/j.cnki.hjwsxzz.2015.04.003
    引用本文: 姜瑞雪, 牛志尊, 黄密, 夏婷婷, 马作峰. 杏苏散、桑杏汤对PM2.5染毒大鼠肺组织中HMGB1、TNF-α、IL-6表达的影响[J]. 环境卫生学杂志, 2015, 5(4): 317-320. DOI: 10.13421/j.cnki.hjwsxzz.2015.04.003
    shu
    JIANG Ruixue, NIU Zhizun, HUANG Mi, XIA Tingting, MA Zuofeng. Effects of Xing Su San and Sang Xing Tang on HMGB1, TNF-α and IL-6 Expression in Rats Lung Tissue Injured by PM2.5[J]. Journal of Environmental Hygiene, 2015, 5(4): 317-320. DOI: 10.13421/j.cnki.hjwsxzz.2015.04.003
    Citation: JIANG Ruixue, NIU Zhizun, HUANG Mi, XIA Tingting, MA Zuofeng. Effects of Xing Su San and Sang Xing Tang on HMGB1, TNF-α and IL-6 Expression in Rats Lung Tissue Injured by PM2.5[J]. Journal of Environmental Hygiene, 2015, 5(4): 317-320. DOI: 10.13421/j.cnki.hjwsxzz.2015.04.003
    shu

    杏苏散、桑杏汤对PM2.5染毒大鼠肺组织中HMGB1、TNF-α、IL-6表达的影响

    Effects of Xing Su San and Sang Xing Tang on HMGB1, TNF-α and IL-6 Expression in Rats Lung Tissue Injured by PM2.5

      摘要
    • 摘要:
      目的 观察杏苏散、桑杏汤对PM2.5致Wistar大鼠肺损伤组织中HMGB1、TNF-α、IL-6表达的影响。
      方法 32只健康SPF级雄性Wistar大鼠随机分为空白对照组、PM2.5组、杏苏散组、桑杏汤组, 每组8只。使用便携式大气细颗粒物PM2.5采样器, 收集武汉工业区大气中的PM2.5, 配制成混悬液, 在模拟秋季雾霾环境下, 经气管滴注对动物进行染毒, 采用杏苏散、桑杏汤, 分别对PM2.5致肺损伤大鼠进行干预。通过检测染毒大鼠肺组织中HMGB1、TNF-α、IL-6的表达水平, 探讨两种方剂对染毒大鼠肺脏的保护作用。
      结果 杏苏散、桑杏汤两组在HMGB1、TNF-α、IL-6的表达水平上与PM2.5组比较, 差异均具有统计学意义 (P < 0.05), 但与空白对照组比较, 三者表达水平接近, 组间比较差异均无统计学意义 (P>0.05)。
      结论 杏苏散、桑杏汤均能显著降低PM2.5致Wistar大鼠肺损伤组织中HMGB1、TNF-α、IL-6的表达水平, 对肺组织具有一定的保护作用, 二者有望成为中医防治雾霾伤肺的有效方剂。

       

      Abstract:
      Objective To investigate the effect of Xing Su San (apricot kernel and perilla powder) and Sang Xing Tang (mulberry leaf and apricot kernel decoction) on the expression of HMGB1, TNF-ɑ and IL-6 in the lung tissue of Wistar rats injured by PM2.5.
      Methods Thirty two healthy male SPF Wistar rats were randomly divided into 4 groups:a control group, a PM2.5 group injured by instillation of PM2.5 suspension, and other two experimental groups treated both by the instillation of PM2.5 suspension and XING SU SAN/SANG XING TANG, respectively. Portable PM2.5 sampler was used to collect atmospheric PM2.5 in Wuhan industrial areas. Collected PM2.5 sample was formulated into a suspension, the concentration of PM2.5 was simulated to the environment for haze in the autumn, and the suspension was injected into rats by intratracheal instillation. The expression of HMGB1, TNF-ɑ and IL-6 in lung tissue was tested to explore the protective effect of XING SU SAN or SANG XING TANG on the lung tissue of rats injured by PM2.5.
      Results The expression of HMGB1, TNF-α and IL-6 in experimental groups, which were treated both by PM2.5 instillation and XING SU SAN/SANG XING TANG, were statistically different from the group treated only by PM2.5 suspension (P < 0.05); however, the expression of HMGB1, TNF-α and IL-6 in experimental groups was not different from the control group (P>0.05).
      Conclusions The injury of PM2.5 on the expression of HMGB1, TNF-α and IL-6 in the lung tissue of Wistar rats could be significantly relived by XING SU SAN or SANG XING TANG, which might have a certain protective effect on the lung injury induced by PM2.5 in rats. These two traditional Chinese prescriptions might be expected to be an effective treatment method for the injury of haze on lung tissue in rats.

       

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