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    付国庆, 石玉琴, 朱丽姗, 唐啸, 芦帷, 张玲, 常薇, 张志兵. 邻苯二甲酸二乙基己酯对小鼠初级精母细胞氧化应激及凋亡的影响[J]. 环境卫生学杂志, 2015, 5(4): 312-316. DOI: 10.13421/j.cnki.hjwsxzz.2015.04.002
    引用本文: 付国庆, 石玉琴, 朱丽姗, 唐啸, 芦帷, 张玲, 常薇, 张志兵. 邻苯二甲酸二乙基己酯对小鼠初级精母细胞氧化应激及凋亡的影响[J]. 环境卫生学杂志, 2015, 5(4): 312-316. DOI: 10.13421/j.cnki.hjwsxzz.2015.04.002
    shu
    FU Guoqing, SHI Yuqin, ZHU Lishan, TANG Xiao, LU Wei, ZHANG Ling, CHANG Wei, ZHANG Zhibing. Effects of Di-2-ethylhexyl Phthalate on Oxidative Stress and Apoptosis in GC-2 spd Cells[J]. Journal of Environmental Hygiene, 2015, 5(4): 312-316. DOI: 10.13421/j.cnki.hjwsxzz.2015.04.002
    Citation: FU Guoqing, SHI Yuqin, ZHU Lishan, TANG Xiao, LU Wei, ZHANG Ling, CHANG Wei, ZHANG Zhibing. Effects of Di-2-ethylhexyl Phthalate on Oxidative Stress and Apoptosis in GC-2 spd Cells[J]. Journal of Environmental Hygiene, 2015, 5(4): 312-316. DOI: 10.13421/j.cnki.hjwsxzz.2015.04.002
    shu

    邻苯二甲酸二乙基己酯对小鼠初级精母细胞氧化应激及凋亡的影响

    Effects of Di-2-ethylhexyl Phthalate on Oxidative Stress and Apoptosis in GC-2 spd Cells

      摘要
    • 摘要:
      目的 研究邻苯二甲酸二乙基己酯 (DEHP) 对小鼠初级精母细胞 (GC-2 spd) 氧化应激及凋亡的影响。
      方法 体外传代培养永生化的GC-2 spd细胞, 用二甲基亚砜 (DMSO) 溶解DEHP, 以DMSO浓度为0.1%的细胞孔作为对照组, 用DEHP终浓度为50、100和200 μmol/L的细胞孔作为低、中、高剂量组染毒24 h。分光光度计法测定GC-2 spd细胞丙二醛 (MDA) 含量, 超氧化物歧化酶 (SOD) 活力和谷胱甘肽过氧化物酶 (GSH-Px) 活力, 流式细胞仪检测不同剂量组细胞凋亡情况。
      结果 与对照组比较, DEHP低、中、高剂量染毒组MDA含量依次升高, 差异无明显统计学意义 (P>0.05); SOD活力在DEHP高剂量染毒组下降, 差异有统计学意义 (P < 0.05); DEHP低、中剂量染毒组GSH-Px活力明显下降, 且差异有统计学意义 (P < 0.05)。DEHP中、高剂量染毒组细胞凋亡明显高于对照组, 且差异有统计学意义 (P < 0.05)。
      结论 DEHP可通过诱发小鼠生精细胞氧化应激而诱导细胞凋亡。

       

      Abstract:
      Objective To explore the effect of di-2-ethylhexyl phthalate (DEHP) on oxidative stress and apoptosis in GC-2 spd cells.
      Methods Immortalized GC-2 spd cells were subcultured and treated with different concentration of DEHP (0, 50, 100 and 200 μmol/L) for 24 h; DEHP was dissolved in 0.1% dimethyl sulfoxide (DMSO). The content of MDA, the activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in GC-2 spd cells were detected by spectrophotometry, and the apoptosis of GC-2 spd cells was analyzed by flow cytometry.
      Results Compared with the control group (0 μmol/L DEHP), the content of MDA was increased with the increase of DEHP, but the difference was not statistically significant (P>0.05). The activity of SOD was decreased significantly in the 200 μmol/L DEHP group (P < 0.05), and the activity of GSH-Px was decreased in the 50 μmol/L and 100 μmol/L DEHP group. The apoptosis of GC-2 spd cells was increased significantly in 100 μmol/L and 200 μmol/L DEHP group (P < 0.05).
      Conclusion DEHP might induce apoptosis in GC-2 spd cells by oxidative stress.

       

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