壬基酚孕期暴露对仔鼠脑皮质尼氏小体的影响
Effects of Nonylphenol (NP) Exposure During Pregnancy on Nissl Body of Rat Cerebral Cortex
-
摘要:目的 检测孕期暴露壬基酚对仔鼠脑皮质尼氏小体的影响。方法 将孕鼠分为4组, 在受孕的8~14 d开始灌胃, 分别为阴性对照组和低、中、高剂量组(壬基酚质量浓度为50、100、200 mg/kg), 在小鼠出生后21、61 d解剖取其脑组织分别做HE染色和改良后的尼氏染色, 观察其结构的组间差异。结果 HE染色显示, 染毒低剂量皮质区神经元开始出现固缩, 随着剂量的升高, 皮质区神经元层次出现紊乱, 排列稀疏散乱, 脱失明显, 细胞体积缩小, 核深染。尼氏染色中, 随着壬基酚质量浓度增加, 尼氏体的数量相对于对照组来说数量依次减少, 且分布不均, 形状大小均不一样, 染色的深浅、透明度也不一致。与对照组比较, 高剂量组光密度有统计学差异(P < 0.05)。结论 孕期暴露壬基酚可导致仔鼠皮质部位神经元出现形态学改变, 随着染毒剂量的增加这种改变越明显, 这可能是壬基酚导致仔鼠学习记忆下降的原因之一。Abstract:Objective To explore the effect of NP exposure during gestation on the Nissl body of cortex in filial rats.Methods regnant rats were treated by gavage with NP at the dosage of 0, 50, 100 or 200 mg/kg each day on the 8th to 14th day after gestation. Filial rats were killed at the 21st and 61st day after birth; brain tissues were stained by HE and modified Nissl method.Results By staining with HE method, the appearance of cortex neurons was shrinking in low NP treated group; and the arrangement of cortex neurons appeared disorder, scattered sparsely, and obvious depigmentation with the increase of NP dosage. The nuclei of neuron in treated groups were smaller and darker than the normal. By staining with Nissl method, the number of Nissl bodies in cortex neurons in treated groups was reduced with the increase of NP exposure in comparison with the control group. The distribution of Nissl bodies appeared uneven, different shapes and inconsistent in the depth of dyeing color and transparency. The optical density of the stained specimen was significantly different between the high NP treated group and the control group (P < 0.05).Conclusions NP exposure during gestation could cause the morphological alteration of cortex neurons in filial rats, these alterations were obvious with the increase of NP exposure, which might be one of the reasons inducing the decrease of learning and memory ability in filial rats.
下载: