高效液相色谱法测定PM<sub>2.5</sub>中多环芳烃

高效液相色谱法测定PM_2.5中多环芳烃

摘要:
目的建立高效液相色谱法测定大气细微颗粒(PM_2.5)中16种常见多环芳烃(PAHs)的最佳分离测定条件。
方法空气中多环芳烃经滤膜收集, 超声提取, 乙腈和水为流动相, 高效液相色谱梯度淋洗分离, 二极管阵列检测器检测。
结果 16种多环芳烃化合物在35 min内得到了很好的分离, 在0.10~5.00μg/mL范围内线性关系良好, 测定的相对标准偏差为1.29%~5.08%, 加标回收率为81.44%~98.02%。
结论该方法测定空气中多环芳烃化合物快速简便、灵敏、重现性好。

Abstract:
Objectives To establish an optimal condition for separating and measuring 16 kinds of polycyclic aromatic hydrocarbons in PM_2.5 by high-performance liquid chromatography(HPLC)with ultraviolet detector.
Methods Polycyclic aromatic hydrocarbons (PAHs) in air were collected by filter membrane and extracted by ultrasonic wave.Using water-acetonitrile as mobile phase, PAHs were efficiently separated by gradient elution and detected by photo-diode-array detector.
Results Sixteen kinds of PAHs were separated efficiently within 35 min and showed good linearity in the range of 0.10~5.00 μg/mL.The relative standard deviations were 1.29%~5.08%, the recoveries were 81.44%~98.02%.
Conclusions PAHs in the air could be analyzed by HPLC because the method was quick, simple, sensitive and reproducible.