高级检索
    马雪莲, 马惠荣, 罗亚萍, 陈景伟, 陈金金. 手机频率辐射对大鼠睾丸形态和Nrf2蛋白表达的影响[J]. 环境卫生学杂志, 2015, 5(1): 10-13. DOI: 10.13421/j.cnki.hjwsxzz.2015.01.003
    引用本文: 马雪莲, 马惠荣, 罗亚萍, 陈景伟, 陈金金. 手机频率辐射对大鼠睾丸形态和Nrf2蛋白表达的影响[J]. 环境卫生学杂志, 2015, 5(1): 10-13. DOI: 10.13421/j.cnki.hjwsxzz.2015.01.003
    shu
    MA Xuelian, MA Huirong, LUO Yaping, CHEN Jingwei, CHEN Jinjin. Effects of Cellphone Frequency Radiation on Morphology and Expression of Nrf2 Protein in Rats Testis[J]. Journal of Environmental Hygiene, 2015, 5(1): 10-13. DOI: 10.13421/j.cnki.hjwsxzz.2015.01.003
    Citation: MA Xuelian, MA Huirong, LUO Yaping, CHEN Jingwei, CHEN Jinjin. Effects of Cellphone Frequency Radiation on Morphology and Expression of Nrf2 Protein in Rats Testis[J]. Journal of Environmental Hygiene, 2015, 5(1): 10-13. DOI: 10.13421/j.cnki.hjwsxzz.2015.01.003
    shu

    手机频率辐射对大鼠睾丸形态和Nrf2蛋白表达的影响

    Effects of Cellphone Frequency Radiation on Morphology and Expression of Nrf2 Protein in Rats Testis

      摘要
    • 摘要:
      目的 观察900 MHz手机频率辐射对雄性大鼠睾丸形态和Nrf2蛋白表达的影响。
      方法 30只SD雄性大鼠随机均分为正常组、12 d及30 d辐射组。12、30 d辐射组每日900 MHz手机频率辐射4 h, 分别连续12、30 d后麻醉大鼠, 取睾丸组织, HE染色观察组织形态, 免疫组织化学法检测Nrf2蛋白表达。
      结果 与正常组相比, 12 d辐射组大鼠睾丸组织形态学变化不明显, 30 d辐射组其组织形态学变化显著:生精小管细胞结构松散, 精母细胞层数减少且出现空洞; 免疫组化结果显示:正常组和30 d辐射组Nrf2蛋白主要表达于精原细胞和精母细胞浆, 12 d辐射组则主要表达于胞核。
      结论 手机频率辐射可造成大鼠睾丸损伤, 12 d可使睾丸Nrf2蛋白出现核转位, 30 d核转位消失。900 MHz手机频率辐射可能通过影响Nrf2蛋白表达, 对雄性大鼠生殖功能造成损伤。

       

      Abstract:
      Objectives  To observe the impact of cellphone frequency radiation on the histological and morphological changes of testis and the expression of Nrf2 protein in the testis of rats.
      Methods Thirty adult male SD rats were randomly divided into three groups:a control group and two radiated groups, which were exposed to a radiation of 900MHz imitated by cellphone in 4 hours a day for 12 days and 30 days respectively. The morphology of testis was examined by routine HE staining, and the expression of Nrf2 protein was detected by immunohistochemical method.
      Results Compared with the control group, there were no obviously morphological change in testis in the 12d-radiated group. The morphological changes in the 30d-radiated group were porosity in seminiferous tubule, loose in the structure of germ cell, and a reduction of spermatozoa.The expression of Nrf2 protein was in the cytoplasm of germ cells in the control group and 30d-radiated group, but it was mainly in nucleus in the 12d-radiated group.
      Conclusions The testis of male rats could be damaged by exposure to a 900 MHz radiation imitated by cellphone. The nuclear translocation of Nrf2 protein was observed in the 12d-radiated group but not in the 30d-radiated group. The testis of male rats could be damaged by an exposure to 900 MHz cellphone radiation, which probably be caused by impacting the nuclear translocation of Nrf2 protein.

       

      • HTML全文
      • 参考文献(17)
      • 相关文章
      • 施引文献
    • 资源附件(0)

    /

    返回文章
    返回