四溴双酚A对HepG2细胞线粒体膜电位及凋亡的影响

向明灯; 李良忠; 玉琳; 于云江

doi:10.13421/j.cnki.hjwsxzz.2015.01.002

四溴双酚A对HepG2细胞线粒体膜电位及凋亡的影响

Effect of Tetrabromobisphenol A on Mitochondrial Membrane Potential and Apoptosis of HepG2 Cells

摘要

摘要:
目的研究不同浓度四溴双酚A(TBBPA)暴露对人肝癌(HepG2) 细胞凋亡的影响。
方法以不同浓度TBBPA染毒HepG2细胞, 采用MTS法检测细胞存活率, JC-1荧光探针检测细胞线粒体膜电位变化, 以AnnexinⅤ与PI联合标记并使用流式细胞仪检测细胞凋亡。
结果 HepG2细胞存活率随着TBBPA暴露浓度升高相应降低; 与对照组相比, 20和30μmol/L TBBPA暴露组HepG2细胞线粒体膜电位显著下降(P < 0.01);HepG2凋亡细胞随TBBPA暴露浓度升高有增加趋势, 其中30μmol/L TBBPA暴露组与对照组相比差异显著(P < 0.01)。
结论 TBBPA可能通过降低线粒体膜电位引起细胞凋亡。

Abstract:
Objectives To investigate the effect of tetrabromobisphenol A on the apoptosis of HepG2 cells.
Methods The viability of HepG2 cells was measured by MTS assay kit, the effect of tetrabromobisphenol A on mitochondrial membrane potential was measured by using a JC-1 fluorescence probe; and the apoptosis of cells labeled with AnnexinⅤand PI was detected by flow cytometry.
Results The viability of cells was decreased with the increase of TBBPA exposure.Comparing with control cells, the mitochondrial membrane potential of cells treated with 20 and 30 μmol/L TBBPA was significantly decreased. The apoptosis of cells induced by TBBPA was increased in a dose-dependent manner, and a statistically significant increase of apoptosis was observed at a concentration of 30 μmol/L.
Conclusions TBBPA could induce an apoptosis of HepG2 cells, which may be related to the decrease of mitochondrial membrane potential.

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